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immunostaining  (Vector Laboratories)


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    Vector Laboratories immunostaining
    Immunostaining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunostaining/product/Vector Laboratories
    Average 96 stars, based on 1121 article reviews
    immunostaining - by Bioz Stars, 2026-05
    96/100 stars

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    Persistent periodontal defects in Hyp groups despite Spp1 ablation. (A) H&E staining shows thin dentin (arrows) and wide pulp chamber compared to WT and Spp1 −/− controls. (B) Picrosirius red (PR) staining demonstrates that all Hyp groups have disorganized and detached PDL fibers at root surfaces (asterisks), compared to controls. (C) Immunohistochemistry (IHC) for osteopontin (OPN) shows accumulation of the protein around the osteocytes in alveolar bone (triangles) of Hyp and Hyp;Spp1 −/+ . Absence of OPN is apparent in Spp1 −/− and Hyp;Spp1 −/− mice. The graph in (D) IHC specific to bone sialoprotein (BSP) reveals unmineralized osteoid areas (asterisks) of alveolar bone that remained unchanged in Hyp groups despite Spp1 −/− deletion. (E) Acellular cementum thickness remains diminished in all Hyp mice compared to controls. (F) Dynamic mechanical analysis testing reveals compromised dynamic stiffness (K * ) and energy dissipation (tan δ) in Hyp mice remain despite Spp1 ablation. AB: alveolar bone; CC: cellular cementum; DE: dentin; PDL: periodontal ligament; AC: acellular cementum. * p < .05; ** p < .01; *** p < .001; **** p < .0001.

    Journal: JBMR Plus

    Article Title: Genetic reduction or deletion of osteopontin in osteomalacic Hyp mice fails to ameliorate dentoalveolar defects

    doi: 10.1093/jbmrpl/ziaf170

    Figure Lengend Snippet: Persistent periodontal defects in Hyp groups despite Spp1 ablation. (A) H&E staining shows thin dentin (arrows) and wide pulp chamber compared to WT and Spp1 −/− controls. (B) Picrosirius red (PR) staining demonstrates that all Hyp groups have disorganized and detached PDL fibers at root surfaces (asterisks), compared to controls. (C) Immunohistochemistry (IHC) for osteopontin (OPN) shows accumulation of the protein around the osteocytes in alveolar bone (triangles) of Hyp and Hyp;Spp1 −/+ . Absence of OPN is apparent in Spp1 −/− and Hyp;Spp1 −/− mice. The graph in (D) IHC specific to bone sialoprotein (BSP) reveals unmineralized osteoid areas (asterisks) of alveolar bone that remained unchanged in Hyp groups despite Spp1 −/− deletion. (E) Acellular cementum thickness remains diminished in all Hyp mice compared to controls. (F) Dynamic mechanical analysis testing reveals compromised dynamic stiffness (K * ) and energy dissipation (tan δ) in Hyp mice remain despite Spp1 ablation. AB: alveolar bone; CC: cellular cementum; DE: dentin; PDL: periodontal ligament; AC: acellular cementum. * p < .05; ** p < .01; *** p < .001; **** p < .0001.

    Article Snippet: Immunohistochemistry (IHC) was performed using an avidin/biotin-based peroxidase immunostaining kit (Vectastain Elite ABC-HRP kit, Vector Laboratories) to produce a red-brown product ( n = 5/group for each target).

    Techniques: Staining, Immunohistochemistry